文档介绍：高效液相色谱法同时测定甘草中甘草酸、甘草苷、异甘草素的含量
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作者:陈云华,赵晓霞,王文全,段天璇
【摘要】目的建立同时测定甘草中甘草酸、甘草苷和异甘草素含量的方法。方法采用高效液相色谱法对甘草粉末的67%甲醇提取物进行分析。用C18反相色谱柱,1%磷酸水-乙***梯度洗脱,对不同色谱峰分别采用248、276、360、370 nm紫外波长检测。结果甘草酸的回归方程为Y=1×106X+16 220,r2=1;甘草苷的回归方程为Y=2×106X+49 444,r2= 5;异甘草素的回归方程为Y=1×107X+ 7,r2=1。甘草酸、%、%、%。结论本方法准确、稳定、可靠,可用于甘草中甘草酸、甘草苷和异甘草素3种成分的同时测定。
【关键词】甘草;甘草酸;甘草苷;异甘草素;高效液相色谱法;含量测定
Abstract:Objective To develop a HPLC quantitatively test method of glycyrrhizin, liquiritin and isoliquritigenin simultaneously for licorice. Methods The 67% methanol extracts of licorice powder were analyzed by reverse-phase HPLC on an ODS column, using a gradient elution with a mixture of 1% H3PO4 and acetonitrile. The eluate was monitored by UV absorption at 248, 276, 360 and 370 nm. Results The linear equation of glycyrrhizin was Y
=1×106X+16 220, r2=1. The linear equation of liquiritin was Y=2×106X+49 444, r2= 5. The linear equation of isoliquritigenin was Y=1×107X+ 7, r2=1. The recovery rate of glycyrrhizin, liquiritin and isoliquritigenin was %, % and % respectively. Conclusion The method is accurate, stable amd reliable, and can be used to determine the three main chemical contents simultaneously.
Key words:Licorice;glycyrrhizin;liquiritin;isoliquritigenin;HPLC;content determination
在甘草质量研究中,建立稳定可靠的甘草