文档介绍：款冬花配方颗粒制备工艺优化及质量控制
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作者:刘可越,汤自豪,刘海军,张铁军,曲伟红
【摘要】目的优化款冬花配方颗粒的制备工艺,建立指标成分芦丁的含量测定方法。方法以正交实验法对款冬花配方水提工艺的影响因素进行考察,优选工艺条件; 用HPLC法测定芦丁含量,检测波长为360 nm ,流动相为甲醇-%磷酸水溶液(50∶50)。结果优选提取工艺为:10倍量水,回流提取3次, h/次。芦丁提取率为85. 53%,%;芦丁在1~ μg(r= 8)范围内呈良好线性关系。%(n=5)。结论款冬花配方颗粒的制备工艺稳定、可行;评价指标可靠、合理。
【关键词】款冬花; 配方颗粒; 芦丁; 质量控制
ChinaAbstract:ObjectiveTo optimize the extraction process for the preparation of Tussilago farfara formula granules and to establish an HPLC method for the determination of rutin in the optimum water extraction process was selected by the orthogonal design and the content of rutin in formula granules was analyzed by HPLC method. The HPLC assay was performed by using MeOH-% phosphoric acid (50
∶50) . The detection wavelength was set at 360 nm. ResultsThe optimum extraction conditions were to reflux for 3 times with 10 folds of water and each time for h. The extraction rate of ratin was 85. 53%, and the dried extraction yield was %. The peak area had a good linearity with the concentration of rutin and the linear range was 1~ μg(r= 8).The average recovery was %(n=5).ConclusionThe preparation process of Tussilago farfara formula granules is stable and feasible. The HPLC method for quality control is accurate and suitable.
Key words:Tussilago farfara; Formula