文档介绍：Introduction
Diagnostic Microbiology: Isolation and Isolation and Culture Procedures
Identification of Pathogens A variety of procedures are employed for the collec-
tion, isolation, and identification of pathogenic
The definitive diagnosis of an infectious disease is in
anisms from different anatomical sites
part dependant on the detection and/or the isolation including tissue, body fluids, pulmonary secretions,
and identification of the pathogenic anism and blood. Various procedures are used for the isola-
or the detection of antigens or antibodies specifically tion of different types of anisms. Labora-
associated with the pathogen. Specific culture meth- tory procedures are designed to screen and facilitate
ods are used for the isolation and identification of a the recovery of etiologic agents of disease that pre-
wide variety of pathogenic anisms. Tradi- dominate in particular clinical syndromes. When the
tional methods for the identification of pathogens manifestation of a disease suggests that the disease
depend on microscopic observations, phenotypic may be caused by a rare pathogen and/or routine
characteristics observed in culture, and metabolic screening fails to detect a probable causative micro-
changes following growth in a variety of substrates. organism, additional specialized isolation proce-
Many differential media and selective media utilize dures may be required.
color changes to highlight specific cultural features As the clinical microbiology laboratory slowly
or substrate utilization. These growth-dependent enters into the new era of “molecular diagnostics,”
methods provide a reliable and relatively accurate the dependency on traditional culture methods
means for identifying the pathogen and diagnosing involving the production and preparation of growth
many infectious diseases. supporting media will continue to play an integral
role in our efforts to detect and characterize both
A wide range of biochemica