文档介绍：f脚删删舢删舢册||J|『舢肼 Y25891 38 Objective Todetermine theeffectofPUFA diet on gutmicrobiota and obesity-related cell factorsin Methods A totalof5weeks old30 healthy female rats were randomly divided intothree groups: controlgroup,n-6PUFA-containing dietgroup andn-3PUFA-containing dietgroup and freefeeding and water foreight serum,body and foodintake were recorded perday during the weight,epididymal fatpadweight,changes ofnumber ofbacteria,thickness of Mucus Layer,and relative expression ofobesity-related cellfactors were levels of Bifidobacterium(Bif),Lactobacillus(Lac),Akkermansiamuciniphila(hkk),Clostridriumdifficile(Clo), us aureus(sta),Ecoli(Eco)and obesity-related cellfactorsFAS,adiponectin,nesfatin-1 and ghrelin were assessed byqRT-PCR method and theprotein levelofnesfatin-1 was assessedby western Results Thebodyweight andepididymal fatpadweight of PUFA group were lowerthan control group,and higher inn- foodintakeWas alsolower in n-3andhigher pared with layer thickness ofn-3group Was significantly higher than controlgroup妒<). Q—PCRanalysis revealed group differences intheBifidobacterium,Lactobacillus,and Akkermansia muciniphila numbers ofwhich weresignificantlyhigher(,P=- andP=-,respectively) m n-3group than incontrol group,whereas Clostridrium difficile us aureusnumbers werelower(P=,).The levelofEcoWas notsignificant difference ). The mRNA levelofFAS and nesfatin-1 and theprotein levelofnesfatin-1 were lower than others inn-3 group,whereas the levelofadiponectin andghrelin were significantly higher than that inthecontrol group∽<).In n·6group,the expression ofnesfatin·l Washigher than control group妒=). Other indicators were nosignificant differences inthe n-6group. Conclusion The resultsprovide auseful resource forfuture studies on the roleofPUFA·modulated position ofgut bacteria which provides anovel way forunderstandi