楸树无性系离体培养特性差异的研究.doc

文档介绍：西北植物学报,2012,32(1):0199—0204
Acta .
文章编号:1000—4025(2012)01一0199—06
楸树无性系离体培养特性差异研究
于永明1,王军辉弘,马建伟1,张宋智1,李平英1,韩云花1,魏秀琴1
(1甘肃省小陇山林业科学研究所,甘肃天水741022;2中国林业科学研究院林业所,国家林业局林木培育重点实验室,北京
100091)
摘

要:对选育的5个楸树无性系(004—1、1-3、2 6、015-1、卜4)进行组织培养比较研究,以明确楸树无性系再生芽增
殖和生根培养中遗传因素及外界条件的影响。结果表明:楸树不同无性系是影响瓶苗生长的主要因素,继代培养
%~%,芽增殖系数、增殖芽数、芽长、叶数、茎段基部愈伤组织横向膨大、茎段
基部愈伤组织纵向膨大在不同无性系间达到极显著水平;生根率、生根数和根长无性系问差异极显著,方差分量分
%、%、%。5个无性系生长性状以004—1表现最好,,%,
%,无性系1 4最弱。
关键词:楸树;无性系;离体培养;方差分析
中图分类号:+2
文献标志码:A
Study

on

Characteristic Differences of Catalpa bungei
Clones in vitro Culture
YU

Yong~ruin91,WANG Jun—hui“,MA Jian—weil,ZHANG

Song-zhil,
LI Ping—yin91。HAN
Yun—hual。WEI
Xiu—qinl
(1
Xiaolongshan Research Institute of Forestry,Tianshui,Gansu 741022,China;2 Research Institute of Forestry,Chinese Acade~
my of Forestry,Key I,ahoratory of
Tree Breeding and Cultivation,State Forestry
Administration,Beijing 100091,China)
Abstract:In order

to

accelerate

propagation of superior clones of Catalpa bungei,tissue culture of five
clones was paratively,which were 004—1,1-3,2-6,015—1 and 1- results showed that the
different clones of Catalpa bungei were the main factors influencing the growth traits of seedling,and the
ponents of subculture were during
%~%.Variance analysis showed that the index
in different clones
were obvious,such
multiplication coefficient,bud number,bud length,leaf number,
callus enlargement of the basal stem,and in rooting culture index such
rooting rate,number
of
roots,root
length,and the ponents were
at
 significant level,and
ponents
were %,%and %, results indicated that 004—1 was the best of the five
clones and the
poorest
 for 004一l,the multiplication coefficient reached ,the rooting
rate
was
%,the transplanting survival
rate
was 74