文档介绍：moncarp鲤鱼grasscarp草鱼tissue组织population群体sexratio性比negative负的positive正的control对照grow生长production生产molecular分子的stock群体sexdeterminationmechanisms性别决定机制superfemale超雌个体progeny后代feeding喂养manipulation管理environment环境study研究species种类catfish鲶鱼microsatellite微卫星elucidate阐明furtherinvestigations进一步的研究significantly显著地spawn产卵expression表达up-icpopulation雌核发育群体thaw解冻fisheries水产trial实验experiment实验storage保存liquidnitrogen液氮waterbath水浴appropriate适宜的condition条件haploid单倍体diploid二倍体hybrid杂交子treatment处理incubator培养容器percentage百分率embryo胚胎fertilizationrate受精率survivalrate成活率data数据batch批randomly随机地sample取样classic经典的mixture混合物enzyme酶Ligation连接Restriction限制性的Dilute稀释pre-icsexing遗传性别鉴定Acknowledgments致谢Mechanism机制Article文章Foundation基础Methylation***化Aromatase芳香化酶Promoter启动子Androgen雄激素Estrogen雌激素Masculinization雄性化inverserelationship负相关suppress抑制genotypicsexdetermination遗传性别决定temperature-dependentsexdetermination温度性别决定thermosensitiveperiod温度敏感期non-ic表观遗传的nuclear细胞核transcriptionfactors转录因子biosynthesis生物合成mutual共同的dinucleotides二核苷酸transcriptionstartsite转录起始位点overall总体上frequencies频率phenotype表型genotype基因型housekeepinggene持家基因exogenous外源的temperature-dependent温度依赖的sex-dependent性别依赖的rear养殖culture养殖stimulate刺激block阻止bioinformatic生物信息的putative推定的co-transfection共转染transcriptionalactivators转录激活因子luciferasereporterassay荧光素酶报告基因检测openingreadingframe开放阅读框similarity相似度development发育demethylation去***化process过程sexdifferentiation性分化silence沉默DNAmethyltransferasesDNA***转移酶Identify鉴定、发现Influence影响Consistent一致的Nutrition营养Disease疾病Ingredient成分bodysize体长polygenic多基因的monosexpopulations单性群体determine确定match匹配consequence结果migration迁移discern辨别hypermethylate超***化phenomenon现象conserved保守的threshold阈值scheme计划FertlizatingrateandsurvialratearetheimportantparametersofrevaluatingThequalityofeggs受精率和成活率是评价卵的重要参数Forimprovingtheculturingproduction,feedingandmanagentisveryimportant饲养的管理对于提高养殖产量非常重要的Thefirststepofbreedingprojectdesignistheselectionofthebasepopulation基础群的选择是育种计划设计的第一步Thetilapiahaveauniquebreedingcharacteristics:Maleterritoryestoblishera