文档介绍：分类号学校代码 10129
U D C 633 学号 08202032

利用 SSR 分子标记划分玉米自交系种质类群的研究
Study on the heterotic group of maize inbred lines with SSR
molecular markers technology

申请人:王宇飞
学科门类:农学
学科专业:作物遗传育种
研究方向:作物抗性、品质、产量等
目标性状的遗传改良
指导教师:郭世华教授

论文提交日期:二〇一一年五月
摘要

本文以引进的 91 份玉米自交系为材料,采用 SSR 分子标记技术进行遗传多样性
和杂种优势类群划分的研究。考虑出苗率、抗病、抗虫抗倒伏等因素,从 91 份引进
的玉米自交系中选取 30 份自交系作为田间试验材料,利用自交系的 10 个性状的田间
调查结果进行方差分析和聚类分析。对形态学方法和 SSR 分子标记类群划分结果进行
了比较,主要结果如下:
20 对 SSR 引物中筛选出多态性较好的引物 6 对。这 6 对 SSR 引物在供试材
料中共检测出 35 个等位基因变异,每对引物检测出的等位基因数在 4—8 个之间,
平均为个, 多态信息量变幅为 -,平均为 ;相异系数变化范围为
-。
SSR 标记,以为阈值将供试 91 份玉米自交系划分为六大类群:第Ⅰ
类群 16 份,第Ⅱ类群 19 份,第Ⅲ类群 7 份,第Ⅳ类群 13 份,第Ⅴ类群 24 份,第Ⅵ
类群 12 份。
SSR-PCR 体系。①:预变性 94℃,4min;②:变性 94℃,
1min;③:退火温度 55-65℃(以引物 Tm 而定),1min;④:延伸 72℃,2min;⑤:
复延伸 72℃,10min;其中步骤 2-4 的循环数为 30-35,以材料而定。
10 个田间性状的调查结果对 30 份材料进行聚类分析,以
为阈值将供试材料分为 6 个类群。

关键词:玉米自交系;SSR 分子标记;聚类分析;杂种优势群
Study on the heterotic group of maize inbred lines with SSR molecular
markers technology

Abstract
y one maize inbred lines in this paper were used to study their ic diversity
and divide heterotic group by SSR molecular marker technology. Based on seedling
percentagy, bisness ressistance, 30 of 91 maize inbred lines were used for variance and
cluster analysis associated with 10 field traits of these inbred lines. the heterotic group
from agronomic pared with that from SSR molecular maekers. The results were as
follows:
1. Six pairs of SSR primers, which had better polymophic, were screened from 20
pairs of SSR primers. The variation of 35 alleles was found out by 6 pairs of SSR primers.
Each pair of primers screened out 4-8 alleles, with the average of alleles for every pair
of primers. The range of polymorphism information contents were -, their average
value was . The Dissimilarity coefficient ranged from to .
2. Based on the SSR finger p