文档介绍：新疆农业大学学报2012,35(2):99~106
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文章编号:1007—8614(2012)02一0099一08
利用SSR标记研究9 6个玉米自交系的
遗传多样性及其群体遗传结构
吉琼1,张新玲2,童婷2,阎晓菲2,张雪妮1,窦秉德3
(,乌鲁木齐830052#,乌鲁木齐
学院植物生物技术研究所,)
830052;
摘要:
分析玉米白交系的遗传多样性,。利用
108对ssR标记引物,对96份玉米白交系进行遗传多样性分析。针对目标群体。使用sTRUcTuRE软件,进行群
体遗传结构评估。设定亚群数目(K)为1~9,并假定标记位点都是独立的,利用△K(E)确定最适合的K值,进而
生成Qmat“x。SsR分析表明,108对SSR引物共检测到472个等位片段。每对引物可以稳定检测到2~9个等
位片段,。群体结构评估表明。最合适的K=2。即目标群体中存在2个
亚群。
关键词:
玉米自交系;遗传多样性;群体结构;微卫星标记
中图分类号:
文献标识码:A
Studies
on
ic Diversity and Population ic
Structure of 96 Maize Inbred Lines Using SSR
Markers
Jl Qion91,ZHANG Xin—lin92,ToNG Tin92,
YAN Xiao—fei2,ZHANG Xue—nil,DOU Bing—de3
( of Agronomy,Xinjiang Ag“cultural University,Urumqi 830052,China; of
Science and
Technology,Xinjiang Agricultural University,Urumqi 830052,China;
Province Key Laboratory for Eco—Agricultural
Biotechnology Around Hongze Lake,Institute of
Plant BiotechnoIogy,Huaiyin Teachers C01lege。Huai’an 223300,China)
AbstI‘act:
This study was carried
analyze the ic diversity of maize inbred 1ines and to study
ic
of germplasm population in order
provide the theoratical basis for preparing dominant
hybrid ic diversity for 96 maize inbred lines were analyzed by using 108 pairs of SSR prim.
population ic
were
assessed
target groups
by using STRUCTURE .
ting subsets(K)for 1—9,using△K(志)to determine the most suitable value of K,and then
generate
the Q analysis showed that 108 primers produced 472 polymorphic ampIi“ed
average
number of alleIes per SSR locus was
with
range
from 2
of population
tion.
showed that the most appropriate K一2,namely,there were 2 sub—groups in the Target popula—
Key words:
maize inbred line;ic diversity;population stru