文档介绍：石河子大学
硕士学位论文
不同剂量甲泼尼龙对大鼠急性百草枯中毒肾脏影响的研究
姓名:豆周林
申请学位级别:硕士
专业:麻醉学
指导教师:马玉英
2011-06
摘要

目的:。
。

方法:根据随机表法,将 120 只 Wistar 大鼠(体重在 200~250g)随机分为五组。空白组、染
毒对照组和甲泼尼龙干预组(根据甲泼尼龙剂量不同再分为三组:①5mg/,②15mg/,
③30mg/),除空白组外,其它各组均予百草枯(22mg/kg)稀释后腹腔注射,2h 后依照
组别、体重注射生理盐水稀释后的甲泼尼龙,在注射甲泼尼龙后的第 24h、72h、168h 共 3
个时间点,按抽签法每组各处死实验对象 6 只获取标本,观察一般情况、肾脏表面及剖面肉
眼变化、肾功能(血尿素氮及血肌酐)和病理变化。

结果:(P=<)和血肌酐(P=<)差异有统
计学意义,干预组中 5mg/kg 甲泼尼龙组同染毒对照组比较血尿素氮和血肌酐显著改善。
(P=<)和血肌酐(P=<)差
异有统计学意义,其中 168h 明显低于 24h、72h。
(P=>)差异无统计学意义。

结论: PQ 中毒大鼠,可以显著减轻 PQ 中毒所致的肾损伤程度,
改善肾功能。
5mg/kg 甲泼尼龙组改善显著,传统的大剂量糖皮质激素冲击治疗
不值的推崇。

关键词:百草枯,中毒,甲泼尼龙,血尿素氮,血肌酐,病理变化

论文类型:B(应用研究)

I
Abstract
Objection: effect of methylprednisolone treatment of renal injury in rats with paraquat
poisoning.
is a reference for the clinical application of GC on paraquat poisoning.
Methods:According to the random table method, 120 Wistar rats (weight200~250g)were
randomly divided into five groups, control group, exposed group and the intervention group
(according to the different doses of methylprednisolone were divided into three groups:
①5mg/,②15mg/,③30mg/), intraperitoneal injection of paraquat(22mg/kg) except
the control group, two hour later,according to group injected with different doses of
,72h,168h in a total of three times, according to the experimental subjects
were killed by drawing of lots 6 from every group to obtain samples of renal function and
pathological changes.

Results:1. Blood urea nitrogen (P=<) and serum creatinine (P=<)in each
group were significantly different;5mg methylprednisolone group was better than the others of the
intervention group,which is significantly different with exposed group.
2.