文档介绍：安徽农业大学
硕士学位论文
贡菊黄酮的提取及对小鼠急性肝损伤保护作用的研究
姓名:王雪
申请学位级别:硕士
专业:生物化学与分子生物学
指导教师:文汉
2011-06
摘要
本实验采用乙醇回流提取法从黄山贡菊中提取粗黄酮,再经过减压抽滤,减压蒸
馏,除去其中的醇类,蒸馏至滤液呈无醇味。再进行一次减压抽滤,接着用氯仿进行
萃取,最后用聚酰胺柱层析进一步纯化贡菊黄酮。真空冷冻干燥获得黄酮粉末,进行
定性分析和定量分析。颜色反应和紫外光谱扫描表明提取物为黄酮类化合物,毛细管
电泳分析表明其主要成分为槲皮素、绿原酸等。定量分析通过芦丁标准曲线的绘制来
计算黄酮的粗提取率,精提取率及黄酮粉末的纯度。
对以四氯化碳造模的急性肝损伤小鼠分别灌胃给予联苯双酯及不同剂量的贡菊
黄酮,采用赖氏法测定血清中丙氨酸氨基转移酶、天门冬氨酸氨基转移酶活性,采用
黄嘌呤氧化酶法测定肝组织中超氧化物歧化酶活性,采用硫代巴比妥酸法(TBA)测定
肝组织中丙二醛含量,并对各组进行比较,观察肝脏病理组织学变化。
实验结果表明:从贡菊中提取的物质符合天然黄酮类化合物的性质,是黄酮类物
质。黄酮的粗提取率为 %,精提取率为 %,黄酮粉末的纯度为 %。对
小鼠急性肝损伤的保护中,各剂量组均能降低急性化学性肝损伤小鼠血清中活性,升
高肝组织超氧化物歧化酶活性,降低丙二醛含量,能不同程度地改善肝脏病理组织损
伤。
关键词:贡菊黄酮,肝损伤小鼠模型,保肝作用,石蜡切片

I
Abstract
The experiment used ethanol refluxing extraction to exact crude flavonoids from
Chrysanthemum, again by the reduced pressure suction filter, vacuum distillation, remove
the alcohol distillation to filtrate is no alcohol taste. For another reduced pressure suction
filter, and then the chloroform extraction, and finally with polyamide column
chromatography, we can get further purification flavonoids from Chrysanthemum.
Vacuum freeze-drying get flavonoids powder, qualitative analysis and quantitative analysis.
Including qualitative analysis is divided into color reaction and UV spectra scanning.
Compared with standard fluid rutin we can determine the extracted from Chrysanthemum
is flavonoids substances. Quantitative analysis through standard rutin curve to calculate the
flavonoids of coarse extraction yield, fine extraction yield and flavonoids powder purity.
To build the model of carbon tetrachloride acute liver damage mice irrigation to the
stomach respectively biphenyl double ester and different doses of flavonoids from
Chrysanthemum, using LaiShi method to determine the serum alanine amino shift enzyme,
aspartic acid amino transfer the yellow enzyme activity method to determine the super
oxide dismutase activity liver ti