文档介绍：NUCLEIC ACIDS / Electrochemical Methods 399
structures of the Drew–Dickerson dodecamer Effect; Multidimensional Proton. Raman Spectroscopy:
(dCGCGAATTCGCG)2 and the DNA hairpin Instrumentation.
d(GCGAAGC) (Figure 7) have been determined with
very high precision using the isotope labeling and the Further Reading
alignment methodology.
Current state-of-the-art NMR technology allows Guschlbauer W (1976) Nucleic Acid Structure. Heidelberg:
studying the fragments of nucleic acids of the size up Springer.
to 70 nucleotides. For molecules with more than 40 Johnson WC (1996) Spectroscopic methods in nucleic acid
studies. In: Fasman GD (ed.) Circular Dichroism and the
residues, the uniform 13C, 15N isotope labeling,
Conformational Analysis of Biomolecules, pp. 433–468.
which, in general, is plicated than in pro- New York: Plenum.
teins, has to plemented also by residue- or Neidle S (ed.) (1999) Oxford Handbook of Nucleic Acid
site-specific labeling. For oligonucleotides with more Structure. New York: Oxford University Press.
than 50 residues partial deuteration seems to be Peticolas WL and Evertsz E (1992) Conformation of DNA
mandatory. Despite these obstacles, the recent im- in vitro and in vivo from laser Raman scattering. In:
provement of labeling strategies, development of new Lilley DMJ and Dahlberg JE (eds.) Methods in Enzymo-
and more sophisticated NMR method