文档介绍：METHODS IN MOLECULAR BIOLOGY TM
Volume 276
Capillary
Electrophoresis
ofof ProteinsProteins
andand PeptidesPeptides
Edited by
MarkMark . StregeStrege
Avinash L. Lagu
CE–ESI/Mass Spectrometry 253
13
Capillary Electrophoresis–Electrospray Ionization Mass
Spectrometry of Amino Acids, Peptides, and Proteins
Mehdi Moini
Summary
Separation in capillary electrophoresis (CE) is based on the movement of -
pounds inside a background electrolyte under an applied potential. Because the mechanism of
separation of CE differs from that of conventional high-performance liquid chromatography
(HPLC), where separation is based on the analyte’s hydrophobic properties, CE is often used as
plementary technique to HPLC. In addition, because CE is performed in narrow capillar-
ies at atmospheric pressure, it is used as an alternative to HPLC, capable of handling small
sample volumes while providing shorter analysis times with higher efficiency. For the analysis
of amino acid, protein, and peptide mixtures in small volume samples such as in single cells,
CE has rapidly evolved as a preferred separation technique. bination of a high-effi-
ciency separation technique, such as CE, with mass spectrometry (MS) detection provides a
powerful system for the analysis plex biological mixtures. In this chapter, a theoretical
and practical approach to achieving high-performance CE–MS is discussed and the utility of
CE–MS for the analysis of amino acids, peptides, and proteins is demonstrated.
Key Words
Amino acids; amino acid enantiomers; capillary electrophoresis; electrospray ionization;
mass spectrometry; peptides; proteins.
1. Introduction
Separation in capillary electrophoresis (CE) is based on the movement of
pounds inside a conductive solution under an applied potential.
Because the mechanism of separation of CE is based on the electrophoretic
mobility of the analytes (which is dependent on the analytes’ charge and shape)
and differs from that of