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第 14 卷第 3 期中国实验方剂学杂志 Vol. 14 ,No. 3
2008 年 3 月 Chinese Journal of Experimental Traditional Medical Formulae Mar. ,2008
蜗牛多糖体外抗乙型肝炎病毒作用研究
湛孝东1 ,王克霞2 ,李朝品1
(1 皖南医学院,安徽芜湖 241002 ; 2 安徽理工大学医学院,安徽淮南 232001)
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[摘要] 目的:探讨蜗牛多糖抗乙肝病毒活性。方法:将不同浓度蜗牛多糖与 HepG2. 2. 15 细胞株共培养,以拉米夫定为
阳性对照药,用 ELISA 法检测 HBsAg 和 HBeAg 分泌水平,实时荧光定量 PCR 检测 HBV DNA 含量,计算抑制率。结果:蜗牛多糖
在体外能有效抑制 HepG2. 2. 15 细胞 HBsAg 和 HBeAg 分泌,最大抑制率分别为 42 8 %和 52 1 % ;对 HBV DNA 的复制有一定抑
制作用( P < 0 05) 。结论:蜗牛多糖在体外具有显著的抗乙型肝炎病毒作用,且毒性较小,具有良好应用前景。
[关键词] 蜗牛多糖;HepG2. 2. 15 细胞;乙型肝炎病毒
[中图分类号] R285 5 [文献标识码] B [文章编号] 1005 9903(2008) 03 0066 04
Studies on Anti HBV Effect in vitro of Snail haride
ZHAN Xiao dong1 , WANG Ke xia2 ,LI Chao pin1
(1. Wannan Medical College , Wuhu 241002 , China ; 2. Medical School ,Anhui
University of Science and Technology , Huainan 232001 , China)
[ Abstract] Objective :To evaluate the anti HBV effect of snail haride(SP) . Methods :Hep G2. 2. 15 cell
strain was used in vitro ,and we applied Lamivudine as positive control. ELISA was adopted to detect the level of HBsAg
and HBeAg in supernatant. The quantity of HBV DNA was checked by RT PCR. The inhibition ratio was calculated of SP
on HBV. Results : SP had a inhibiting effect on the HBsAg and HBeAg with maximum inhibition ratio adding up to
42 8