文档介绍：公司理念：毅新兴业以生命科学研究为本，致力于科学技术服务，为客户提供完美的科研平台。
基因组
SNPs（包括芯片、massarray）
第二代大规模测序
病毒包装
miRNA表达谱
蛋白组
血清多肽谱
2DE
LC-MS（Shotgun法）
代谢组
NMR
LC-MS
自主仪器
恒温金属浴
梯度PCR仪
恒温振荡仪
Squenom质谱仪
SAI质谱仪
MALDI TOF/TOF
测序仪：Polonator
试剂
自主研发
液体蛋白磁珠
SBI试剂
慢病毒
RNA干扰库
MicroRNA研究
干细胞研究
二代测序简介
2021/1/11
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内容提纲
从Sanger法到新一代测序技术
第二代测序平台介绍
第二代测序技术应用
宏基因组学（Metagenomics）
泛基因组学（Pangenomics）
二代测序简介
2021/1/11
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Key Genomics Technologies
1975 - Southern DNA hybridization technique
1977 - Sanger’s chain-termination and Maxam、Gilbert’s chemical DNA sequencing methods
1980 - Automated in situ oligonucleotide synthesis instrument
1985 - Mullis’s discovery of PCR at Cetus
1992 - Affymatrix (Fodor’s group) first gene-chip
1995 - ABI’s first automated DNA sequencer
2006 - 2nd generation DNA sequencer on market
2007 and beyond – Single molecule sequencing techniques
二代测序简介
2021/1/11
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Sanger’s Method
Labeled primer (1)
Sequence reactions (4)
Sequence separations (4)
Sequence read-out
二代测序简介
2021/1/11
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1st-Generation Automated DNA Sequencer
Parallel runs on 96 capillaries on ABI 3700
二代测序简介
2021/1/11
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Limitation of 1st Gen Sequencer
Throughput
Time-consuming separation of chain-terminated fragments
Hard to produce massively parallel system based electrophoretic separation
Sequencing Cost
Sample handling
Difficult to miniaturize
二代测序简介
2021/1/11
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Need for Faster and Cheaper Sequencing Technology
Personal Genome Project
二代测序简介
2021/1/11
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Trends in Next-Generation Sequencing
Large-scale and high-throughput
Amplification on solid surface
Cluster generation (bridge or polony amplification )
Emulsion PCR (fragment on beads)
In situ sequencing by chain extension
Sequence by synthesis – DNA polymerase
Sequence by ligation – DNA ligase
Massively parallel processing
Array of clusters, beads
Miniaturi