文档介绍：S07100901 Molecular Biology of the Cell Part II Instructor: 温龙平 ******@ustc.
Topics
Cancer
Stem Cells
Biomembranes
Nanobiology
Grading
Class Attendance & Discussion (20%)
Team Presentation (40%)
Final Exam (40%)
Presentations
5-member team (self-assembled)
Select one paper (Science, Nature, Cell) within the fields of cancer, stem cell or apoptosis, discuss extensively by the team, and prepare for a 20-minute presentation (10-15 slides)
Address the following in your presentation:
Scientific question the paper was trying to answer;
Experimental approaches taken and main techniques used;
Major findings and conclusions;
Significance of the paper;
Your ideas to further the research.
o & Cancer
Cancer: The Bottom Line
In animals, cell numbers are under exquisite control
Loss of this control leads to proliferation of unwanted cells
Cancer arises when the above situation reaches a certain degree
Cancer vs Tumor
Cancer cells are malignant tumor (vs. benign tumor) cells
All cancer cells are eventually capable of metastasis
Tissue Culture Systems
Primary Cell Cultures
Secondary Cell Strains
Continuous Cell Lines
Transformed Cells
Reduced cell adhesion
Round-up morphology
Reduced serum requirements
Elevated hexose uptake
Anchorage-independent growth (ability to grow in semi-solid media,soft agar
Loss of density-dependent growth inhibition (“Contact Inhibition”)
Secretion of plasminogen activator
Capable of forming tumor in appropriete setting
Causes of Cancer Generalizations
Spontaneous ic mutations
Inherited cancer susceptibilities
Carcinogenic substances
Viruses
Radiation