文档介绍：Biotechnology Letters (2005) 27: 641–647 Ó Springer 2005
DOI -005-3660-2
Cloning and expression of the gene encoding Streptomyces coelicolor
A3(2) a-galactosidase belonging to family 36
Kenji Kondoh1, Kohei Morisaki1, Wook-Dong Kim2, Gwi-Gun Park3,
Satoshi Kaneko2 & Hideyuki Kobayashi2,*
1Graduated school of Life and Environmental Science, University of Tsukuba, Ibaraki 305-0006, Japan
2Biological Function Division, National Food Research Institute, Tsukuba, Ibaraki 305-8642, Japan
3Departmant of Food Engineering and Biotechnology, Kyouguan University, Kyunggi-do 461-701, Korea
*Author for correspondence (Fax: +81-29-838-7996; E-mail: ******@)
Received 16 December 2004; Revisions requested 10 January 2005; Revisions received 25 February 2005; Accepted 28 February 2005
Key words: family 36, a-galactosidase, glycoside hydrolase, oligosaccharides, Streptomyces coelicolor
A3(2), substrate specificity
Abstract
The a-galactosidase gene of Streptomyces coelicolor A3(2) was cloned, expressed in Escherichia coli and
characterized. It consisted of 1497 nucleotides encoding a protein of 499 amino acids with a predicted
molecular weight of 57,385. The observed homology between the deduced amino acid sequences of the
enzyme and a-galactosidase from Thermus thermophilus was