文档介绍：沈阳药科大学
本科毕业论文
论文题目：化合物A联合化合物 B对人肝癌HepG2细胞作用机制研究文档来自于网络搜索
起止时间：2014 年10月26日—2015年6月20日个人收集整理勿做商业用途
姓名学号：
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指导教师：XXX 副教授文档来自于网络搜索
实****单位：XXX大学生命科学与生物制药学院药理系文档收集自网络，仅用于个人学****br/>目录
中文摘要······································································1英文摘要······································································2个人收集整理勿做商业用途
第1章绪论··································································3文档收集自网络，仅用于个人学****br/> ·····························································3文档来自于网络搜索
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第2章实验部分······························································6文档收集自网络，仅用于个人学****br/>·····························································6个人收集整理勿做商业用途
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第3章结论与讨论···························································
17文档收集自网络，仅用于个人学****br/>································································17资料个人收集整理，勿做商业用途
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摘要
原发性肝癌是世界常见的恶性肿瘤之一，也是我国的高发恶性肿瘤，由于不易被早期诊断，进展迅速，术后容易复发、转移，因此预后较差。目前肝癌仍以外科手术为公认首选和主要的治疗手段，但由于很多肝癌患者确诊时已是中晚期，失去了手术治疗的机会并于诊断后1年内死亡，手术切除率<30%，术后总体5年生存率仅30%~40%。对于可手术的肝癌患者，术后复发、转移是影响治疗效果及预后的主要因素，对于不能手术的中晚期肝癌患者尚缺乏有效的治疗方法。传统的化疗、放疗对肝癌疗效欠佳，目前仍缺乏行之有效的化学预防和治疗靶位
[1]。因而本课题是对化合物 A联合化合物 B对人肝癌HepG2细胞作用机制研究。文档收集自网络，仅用于个人学****br/>本实验首先用MTT法分别测得不同浓度的A和B单独处理HepG2细胞及不同浓度的A和B联合用药处理HepG2细胞死亡的影响。结果表明不同浓度的A和B单独处理HepG2细胞，增值抑制程度表现为时间剂量依赖效应，并且在A 6μmol/L联合B 100μmol/L协同作用最为显著。流式细胞仪检测细胞凋亡率显示，联合用药组凋亡率升高更为明显。Western Blot法检测蛋白显示，促凋亡相关蛋白Bax、FADD增加，抗凋亡蛋白Bcl-2 表达减少。