文档介绍：SPECTROSCOPY Spectral Distribution of Radiant Energy   Wave Number (cycles/cm) V = Wave Number (cm-1) l = Wave Length C = Velocity of Radiation (constant) = 3 x 1010 cm/sec. u = Frequency of Radiation (cycles/sec)   The energy of photon: h (Planck's constant) = x 10-27 (Ergsec) C = u SPECTROSCOPY DISPERSION OF POLYCHROMATIC LIGHT WITH A PRISM Prism - spray out the spectrum and choose the certain wavelength (l) that you want by slit. SPECTROSCOPY 1. Spectrophotometer - an instrument which can measure the optical density of a sample at any wavelength. 2. Fluorometer - measures the intensity of fluorescent light emitted by a sample exposed to UV light under specific conditions. Fluorometer BEER LAMBERT LAW As the cell thickness increases, the intensity of I (transmitted intensity of light ) decreases. R- Transmittance R = I0 - original light intensity I- transmitted light intensity   % Transmittance = 100 x
Absorbance (A) or optical density (OD) = Log = Log = 2 - Log%T Log is proportional to C (concentration of solution) and is also proportional to L (length of light path through the solution). I I0 I I0 I0 I 1 T I I0