文档介绍：Vll
Preface
NMR has e the most diverse spectroscopic tool available to
date in biomedical research. It is now routinely used to study biomolecu-
lar structure and dynamics particularly as a result of the recent devel•
opments of a cascade of highly sophisticated multidimensional pulse
sequences, and of advances in ic engineering to produce bio-
molecules, uniformly or selectively enriched with ^^C, ^^N and ^H. This
book, written by acknowledged experts, provides an up-to-date treat•
ment of the current status of multi-dimensional NMR, including the
basic aspects, and its application to problems of biomedical interest.
William Westler, in Chapter 1, provides a practical introduction to
two-dimensional NMR through coherent work. This description
makes it easy to conceptualize the pulse sequence details and provides
a basis for understanding plicated sequences. In Chapter 2,
Luciano Mueller and N. Vasant Kumar describe the current status of
multi-dimensional NMR and its utility in structure determination of
proteins and nucleic acids. In addition to a brief description of different
classes of multidimensional experiments they also summarize the spec•
trometer requirements for implementing these sequences.
Because of the recent developments in molecular biology techniques
for preparing labeled samples, it is now possible to perform NMR
studies on large macromolecules. An overview of different procedures
for isotopic enrichment of proteins by labeled amino acids is given by
Brian Stockman in Chapter 3. A critical discussion of the advantages
and disadvantages of different procedures is also provided. Chapter 4,
by Paul Weber, is a critical survey of a number of methods used for
structure calculation of proteins from NMR data and provides the user
with an insight into the process. This chapter also provides a list of
different options available for structure calculation.
In order to unders