文档介绍：ThispageintentionallyleftblankAddresseditorialcorrespondencetoASMPress,,Washington,DC20036-2904,USASendorderstoASMPress,,Herndon,VA20172,USAPhone:(800)546-2416or(703)661-1593Fax:(703)661-1501E-mail:******@Online:©1994,1998,2003,,DC20036-2904LibraryofCongressCataloging-in-PublicationDataGlick,:binantDNA/,,.—.;-1-55581-498-4(hardcover),,.[DNLM:]’5—dc222009026838**********AllRightsReservedPrintedinCanadaCoverandinteriordesign:SusanBrownSchmidlerCoverillustration:TereseWinslowInmemoryofLiliPasternak(1938–2008),mercializationofMolecularTechnology47Biotechnology6RestrictionEndonucleases49ChemicalSynthesis,ConcernsandConsequences10PlasmidCloningVectors57Amplification,andsummary12PlasmidCloningVectorpBR32259SequencingofDNA98references13TransformationandSelection60ChemicalSynthesisofDNA98reviewQuestions13OtherPlasmidCloningVectors63ThePhosphoramiditeMethod99CreatingandScreeningaLibrary68UsesofSynthesizedMakingaGenomicLibrary68Oligonucleotides103chapter2ScreeningbyDNAHybridization70PolymeraseChainReaction108ScreeningbyImmunologicalAssay76PCRAmplificationofFull-LengthDNA,RNA,andProteincDNAs113ScreeningbyProteinActivity78Synthesis14GeneSynthesisbyPCR113CloningDNASequencesThatEncodeStructureofDNA14EukaryoticProteins80DNA-icInformation:RNADNA86SequencingDNA118PrimerWalking124andProtein20BacteriophageλVectors86Pyrosequencing125Translation26Cosmids90SequencingUsingReversibleChainRegulationofmRNATranscriptioninHigh-icTransformationofEukaryotes37Prokaryotes92Large-