文档介绍：Research About A bnormal R eaction of springs bacteria to pre- earthquake in North T ianshan region A Dissertation Submitted to Shihezi University In Partial Fulfillment of the Requirements for the Degree of Master of Natural Science By Luo Jiao ( Biochemistry and Molecular Biology ) Dissertation Supervisor: Lou Kai June , 20 14 万方数据万方数据摘要目的: 为探索一种基于细菌群落的地震监测新技术。本研究以北天山地区用于地震监测的典型泉点(乌鲁木齐 10 号泉)为研究对象,基于震中距乌鲁木齐 10 号泉 100 Km 范围内发生的有感地震, 利用3 种不同技术方法研究乌鲁木齐10 号泉泉水细菌群落对有感地震的映震规律及敏感菌群的代谢特征, 并分析各技术用以地震监测的实效性。方法: 采用传统的纯培养平板计数方法和 BIOLOG GEN III 细菌板从细胞水平上监测地震前后泉水中细菌群落活性及功能多样性变化;利用 T-RFLP ( 末端限制性片段长度多态性) 技术从分子水平上分析地震前后泉水细菌群落结构组成变化, A (典型相关性分析)分析泉水细菌群落与水文理化指标之间的相关性。结果: 泉水细菌群落全年呈现随机动态变化,而震后可培养细菌菌落数目与细菌群落对碳源利用的平均颜色(AWCD) 变化均高于震前,且表现与震级有一定相关性;泉水中对有感地震响应灵敏的细菌类群以糖醇类碳源为主要代谢碳源。 GEN III板中 71 种碳源中的 6 种能够显著体现地震前后水体细菌群落结构的差异, 它们分别为甘油、氨基乙酰-L- 脯氨酸、奎宁酸、 L- 乳酸、丙酸和乙酸, 地震前后判别函数的判别正确率为 % 。在泉水细菌群落组成上, 多数震前细菌群落多样性高于震后,少数震后细菌群落多样性高于震前,这表现泉水细菌群落与水文理化指标、地震震级和泉水补水量有一定相关性;地震震级越大则地震前后泉水中出现的细菌新类群越多;在相近震级下,震源距地面深度越深地震前后泉水中出现的细菌新类群越少;地震后泉水中出现的细菌新类群丰富度大于地震前;地震前后泉水中一些优势类群消失; CCA 分析表明, 水氡(Rn) 是泉水中地震前后出现细菌新类群的主要生长促进因子, 硫化物( HS ) 是主要生长抑制因子。结论: 细菌群落的碳源利用及可培养菌落数目具有一定映震规律, BIOLOG 技术用于北天山地区地震前兆的监测统计准确率提高到 5 %, T-RFLP 技术可发现在地震前后才存在的细菌新类群。关键词: 细菌,乌鲁木齐 10 号泉,平板计数, BIOLOG , T-RFLP 万方数据 Abstract Objective: To explore a kind of new technology of earthquake monitoring based on munity. C onsequently ,t ypical monitoring spring spot from northern Tianshan area for earthquake was Spring in Urumqi that were used to study their reflecting earthquake law of munity and m etabolic characteristics of sensitive bacteria for felt earthquake using three different methods , and analysis of the effect of these methods, water samples were collected from m onitoring period which felt earthquakes occurred and the epicenter was 100 kilometers away from the Spring in Urumqi. Methods: On the one hand , we used p late culture counting method s and BIOLOG GEN IIIb acteria plate to monitor ed bacterial activity and functional