文档介绍：中国农业科学 2010,43(14):
Scientia Agricultura Sinica
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不同耐氮肥烤烟品种质外体 NH4 浓度差异和有关生理指标分析
刘化冰,杨铁钊,张小全,段旺军,尚晓颍
(河南农业大学烟草学院,郑州 450002)
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摘要:【目的】研究不同耐氮肥烤烟品种叶片质外体 NH4 浓度差异与耐氮肥性关系,为筛选对氮素适应范围
广的烤烟品种提供理论依据。【方法】以耐氮肥的烟草品种中烟 90、中耐氮肥的 K326 和对氮肥敏感的 NC89 为试
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验材料,用盆栽法研究它们之间的质外体 NH4 浓度差异。【结果】生育后期 3 个烤烟品种叶片质外体的 NH4 浓度均
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呈现逐步升高的趋势,中烟 90 的质外体 NH4 浓度最大,NC89 (GS)活性和总氮含量下降明显,
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质外体 pH 值急剧减小,谷氨酸脱氢酶(NADH-GDH)活性缓慢上升,质外体 pH 始终低于叶片 pH;质外体 NH4 浓度
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与 GS 活性和总氮都呈显著负相关,与 GDH 活性呈显著正相关。【结论】烤烟品种耐肥性与其生育后期质外体 NH4
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浓度,GS 活性和 GDH 活性有关,耐肥性强的烤烟品种其质外体 NH4 浓度高,GS 活性低,GDH 活性高,NH4 挥发多。
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关键词:烤烟;耐氮肥;质外体 NH4 浓度
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Difference of Apoplastic NH4 Concentration in Flue-Cured
o Varieties with Different Tolerance to Nitrogen Nutrition and
Assay of the Related Physiological Parameters
LIU Hua-bing, YANG Tie-zhao, ZHANG Xiao-quan, DUAN Wang-jun, SHANG Xiao-ying
(College of o, Henan Agricultural University, Zhengzhou 450002)
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Abstract: 【Objective】 The objective of this study was to elucidate the relationship between the apoplastic NH4 concentration
and the nitrogen tolerance of flue-cured o varieties and to provide a theoretical basis for selecting o variety with
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different tolerance to nitrogen nutrition. 【Method】 A pot experiment was carried out to study the difference of apoplastic NH4
concentration in three flue-cured o varieties, ZY90 (tole