文档介绍：摘要本研究采集了呼和浩特地区奶牛流产胎儿的病料及牛乳共54份,并进行了细菌的分离与鉴定。采用细菌分离培养、染色镜检及生化试验等方法,共分离出布鲁氏菌 ll株,其中,从6份流产胎儿病料中分离到5株布鲁氏菌,从48份牛乳中分离到6 株布鲁氏菌。采用二氧化碳试验,硫化氢试验、染料抑菌试验和单相特导性血清A、 M和R玻片凝集试验方法对11株分离菌进行了种型鉴定,证明5株菌为牛种生物3 型布鲁氏菌,另6株菌为猪种生物l型布鲁氏菌。,结果表明6株布鲁氏菌为猪种生物l型,与传统方法的鉴定结果完全一致。本项目研究结果显示,目前呼和浩特地区的奶牛流产主要是由布鲁氏菌病引起的,这一地区布鲁氏菌感染较为严重。关键词:布鲁氏菌病;布鲁氏菌:分离鉴定; AMOS·PCR;生物型 IsolationandIdentification ofBrucella intheRegion ofHuhhot Abstract 54samples ofaborted fetus and milk were collected inthe region ofHuhhot and Brucella were isolated isolatingculture、microscope observation and biochemistry experiment,11 strainsof brucella were strainsof brucella were isolatedfromthe 6samples ofaboaed fetusand 6strainsofbrucella were isolated from the 48samples ofI试u【.By carbon dioxide experiment、hydrogen sulfideexperiment、dye bacteriostasisexperiment and monophase specificity serumA、M、R slideagglutination experiment,it was identificated that5strainsofisolates werethebiotype 3of cattlespecies and theother 6strainswere thebiotype 1ofswine strainsofswinespecies were conformed further by theAMOS-PCR detecting Was revealed thatthe abortion of cow was caused mainly by brucellosis atthepresent time ahdthe bruceila infection wasseriously intheregion ofHuhhot. Key words:Brucellosis;Brucella,Isolation andIdentification;AMOS-PCR Biotype Directed by: ZhiYi() 0ij in() App eant for Master degree:YAO dng(preventiveveterinary) (Animal Science andAnimal Medicine。Inner Mongolia AgriculturalUniversity,Huhhot 010018,China) 内蒙古农业大学研究生学位论文独创声明本人申明所呈交的学位论文是我本人在导师指导下进行的研究工作及取得的研究成果。据我所知,除了文中特别加以标注和致谢的地方外,论文中不包含其他人已经发表或撰写过的研究成果,也不包含为获得我校或其他教育机构的学位或证书而使用过的材料,与我一同工作的同志对本研究所做的任何贡献均已在论文中作了明确的说明并表示谢意。申请学位论文与资料若有不实之处,本人承担一切相关责任. 论文作者签名:埠日期: 、阳内蒙古农业大学研究生学位论文版权使用授权书本人完全了解内蒙古农业大学有关保护知识产权的规定,印:,发表论文或使用论文工作成果时署名单位为内蒙古农业大学,且导师为通讯作者,通讯作者单位亦署名为内蒙古农业大学。学校有权保留并向国家有关部门或机构送交论文的寥印件和电子文档,