文档介绍：表面活性剂论文生物表面活性剂论文正交优选表面活性剂提取山楂黄酮的工艺研究[ 摘要] 目的: 优选用表面活性剂提取山楂中黄酮的最佳工艺条件。方法: 以总黄酮含量为指标,采用单因素试验和正交试验确定最佳提取工艺。结果:山楂中黄酮的最佳提取工艺条件为,采用质量分数为 % 的吐温 80 水溶液,在 90 ℃提取 1h ,提取 2 次,料液比为 1∶ 20 ,提取率为 % ,比传统的乙醇回流提取山楂黄酮的提取率增加了 % 。结论: 该法以溶有少量表面活性剂的水替代高浓度的醇进行活性成分的提取,能大大降低提取成本,并提高提取率, 是较理想的一种提取新工艺。[ 关键词] 表面活性剂;山楂;黄酮;正交试验 Optimum flavonoids extraction from hawthorn fruits with surfactant PAN Fangfang, GAO Jia, CHEN Jing (College of Bioengineering, Zhejiang Chinese Medical University, Hangzhou 310053, China) [Abstract] Objective: To optimum extraction technology of flavonoids from hawthorn fruits with surfactant. Methods: Single factor and orthogonal test were used to study the extraction conditions to extract flavonoids from hawthorn fruits. Results: The optimum extracting condition of extracting flavones from hawthorn was showed as follows, mass fraction of Tween 80 solution %; temperature of estraction 90 ℃; time of extraction 1 h; twice and the solid- liquid ratio 1∶ 40 (g/ml). Conclusion: The method is low cost, high yeild and no need anic solvent and can be applied multipurposely. [Key words] Surfactan; Hawthorn; Flavones; Orthogonal test 山楂为蔷薇科植物山楂( Crataegus Pinnatifida Bunge )、山里红( Crataegus pinnatifida Bunge . )及野山楂( Crataegus cuneata Sieb et . )的干燥成熟果实。山楂性酸、甘、味温,归脾、胃、肝经,具有消食化积、活血化瘀的功效。山楂含有丰富的黄酮类化合物,对心脑血管疾病有显著疗效,具有降血脂、抗心肌缺血及再灌注损伤、增加冠状动脉流量等作用[1] 。山楂黄酮的提取主要有有机溶剂浸提、