文档介绍：2002年第 60卷第 4期, 715~ 719 化学学报 ACTA CHIMICA SINICA Vol . 60 , 2002 No. 4 , 715~ 719 生物酶振荡器研究谷胱甘肽的抗氧化效应甘南琴 蔡汝秀Ξ 林智信 杨树涛(武汉大学化学系武汉 430072) 摘要组装了一种生物酶振荡器(Peroxidase2 Oxidase Biochemical Oscillator )的实验装置、建立了相应的实验方法,在此基础上得到单倍周期、四倍周期、混沌等丰富的振荡图谱信息,并采用它作为“探针”,检测了谷胱甘肽(GSH )的抗氧化效应,它可作为定量分析的基础,线性范围为 9. 38× 10 - 7~ 7. 50× 10 - 5mol· L - 1,探讨了某些可能的反应机理. 关键词生物酶振荡器,PO振荡反应,谷胱甘肽 Study on the Antioxidation Effect of Glutathione Based on the Peroxidase2 Oxidase Biochemical Oscillator GANG, Nan2 Qin CAI , Ru2 Xiu Ξ LIN , Zhi2 Xin YANG, Shu2 Tao (DepartmentofChemistry,WuhanUniversity,Wuhan430072 ) plex dynamics have e increasingly important in biochemical and physiological contexts in recent years. A good example for studying the nonlinear and non2 equilibrium dynamics is the peroxidase2 oxidase (PO )reaction. The PO reaction involves the oxidation of reduced nicotinamide adenine dinucleotide (NADH )by molecular oxygen and overall reaction catalyzed by horseradish peroxidase (HRP ). The non2 linear behavior of this reaction is usually realized in a reactor containing the HRP , methylene blue (MB ), and 2 ,42 dichlorophenol (DCP ). NADH and O 2are supplied continuously to this reaction mixture fromexternal sources. In this paper , the experimental system and methods of the Peroxidase2 Oxidase biochemical oscillator has been set up essfully. Different kinds of oscillating waves such as simple