文档介绍:HPLC法测定黄芪中的黄芪甲苷含量
摘 要:,用色谱纯甲醇溶解并稀释,采用YWG-C18柱,流动相为甲醇,在205 nm下检测, AUFS,%,%,结果表明,~ mg/mL之间与峰面积成良好的线性关系.
High-performance Liquid Chromatographic Determination of
Astragaloside in Astragalus membranaceus(Fisch.)Bunge.
Tian Feng,Deng Yingjie
(Department of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110015)
Abstract:A sensitive method was developed to determine astragaloside by high-performance liquid chromatography with the C18 mobile phase was methanol and the UV absorbency was monitored at 205 assay demonstrated that astragaloside had adequate sensitivity and selectivity to measure the concentrations of astragaloside in Astragalus membranaceus(Fisch.)Bunge.
Key words:high performance liquid chromatography;astragaloside;Astragalus membranaceus(Fisch.)Bunge.▲
黄芪为常用中药,素有“补药之长”之称,具有益气固表,,临床上常用来治疗非特异性免疫功能低下、,其化学结构如图1所示,它具有降压、抗炎、稳定红细胞膜、提高血浆cAMP含量,促进小鼠再生肝DNA合成和增强免疫功能等多种作用〔1〕〔2〕和香草醛-硫酸比色法〔3〕,前者操作繁琐,背景干扰多,误差较大;后者空白吸收值高,受时间因素干扰大,~ ,该法灵敏度高,重现性好,可准确地测定东北黄芪甲苷的含量.
The formulation of astragaloside
1 仪器与方法
仪器:Waters高效液相色谱仪,510型泵,490E紫外检测仪.
对照品 黄芪甲苷购自中国药品生物制品检定所.
样品 黄芪饮片(生品),购自沈阳京盛药店,本校许春泉教授鉴定为东北黄芪.
试剂 乙腈(色谱纯,天津四友生物医学公司)、甲醇(色谱纯,天津四友)、正丁醇(分析纯)、乙醚(分析纯)、水、重蒸馏水、去离子水、中性氧化铝(层析用)、轻