文档介绍：Volume 309, number 3, 343-349 FEB$1153 i ~ptcm~rl~2
O 1992 Federatim~ of Euro~an Biochemical ,¢m¢ietics O014~
Cyclic AMP-dependent phosphorylation and regulation of the cardiac
dihydropyridine-sensitive Ca channel
Akira Yoshida", Masami Takahashi", Seiichiro Nishimura ~', Hiroshi Takeshima t~and Shinichiro Kokubun'
"Mita'ubishi If axeS b~stituw of life Scicnccx, Machldct, Tck),o 194, .tapu., "Dcparuucnt of Medical Chetnlstr¥, Kyata University,
Faculty of Medicine. lfyoto 60¢$. Japan and "Drpartntcnt of Physiola&)', Nihon University School of Medicine, Tokyo 173, ***@an
R¢~ived 5 Sune 1992
A polyclonal antibody, CR2, prepared using the ~inal peptid¢ of the a I subunit of the rabbit cardia~ DHP-~nsitive Ca channel, sp~ill~lly
immunapr¢cipitated tl~¢ [~H|PN200. I i ¢l~ Ca eltan~l so!abilizcd from cardiac microgram, The antibody rcco~iz~d 250 and 200-kDa c~rdhl¢
mi~:ro¢omal prnteins ;tx determined by immunoblotting, anti protein kina~ phosphorylated ~, but not the
protein in vitro. CHO cells, tramll~cted with the ~tdia¢ ;.~ subunit eDNA carried by an ~pr¢~ion v~:tor, synth~'~i~:d a protein which
was recognized by CR2, Adding db-¢AMP or for~kolin to the transformed CHO cells ind