文档介绍：M2 medium NaCl, g KCl, g CaCl 2? 2H 2 O, g KH 2 PO 4, g MgSO 4? 7H 2 O, g NaHCO 3, g HEPES, g Sodium lactate, g (or g of 60% syrup) Sodium pyruvate, g Glucose, 1g Bovine serum albumin (BSA), 4g Penicillin G (potassium salt), g Streptomycin sulfate, g Phenol red, g H 2 O, 2x glass-distilled, to1 liter Note: For Ca 2+ -free medium, omit the CaCl 2 and increase the NaCl to g. Note: The concentration of phenol red can be decreased to - g/liter, as it may be embryo-toxic. 1. Dissolve HEPES in 50-100 ml of 2x distilled H 2 O. 2. Adjust the pH to with M NaOH. 3. Weigh the penicillin and streptomycin and dissolve them ina small volume of 2x distilled H 2 O. 4. Weigh the CaCl 2 and dissolve it in 2x distilled H 2 O. 5. Weigh the ponents (except BSA and lactate) into a designated 1-liter volumetric flask and add approximately 500 ml of 2x distilled H 2 O. Allow to dissolve. 6. Add the penicillin, streptomycin, HEPES, and CaCl 2 toa volumetric flask. 7. Weigh the lactate syrup into a designated 10-ml beaker and add it to the volumetric flask. Rinse the beaker several times with 2x distilled H 2 O, add the washin