文档介绍：Cloning of Upstream Region of cyp51 gene from
Ustilago maydis and Analysis by Bioinformatics
Chen Li1*, Shuxiang Li 1*, Liling Yuan 1, Yi Liu 1, Jiangke Yang 2, Yunjun Yan 2**
Xueting Yang 1, Yongze Yuan1, Deli Liu 1** 2. College of Life Science and Technology ,Huazhong
1. Hubei Key Laboratory of ic Regulation and University of Science and Technology,Wuhan,China ,430074
Integrative Biology ,College of Life Sciences, Central China (*Contributed equally; **Corresponding author E-mail:
Normal University, Wuhan, China,430079 deliliu2002@)
Abstract—CYP51 of Fungi, known as sterol As Ustilago maydis is one of the important anism to study
14-α-demethylase (P450 14DM) is a key enzyme in the the mechanism of resistance owned by filamentous fungi, it is very
biosynthesis of membrane ergosterol. It will lead to the significant to master the upstream regulatory sequence of Ustilago
destruction and loss of function of membrane structure and maydis cyp51 gene and its mechanism of resistance [5].
ultimately to fungal death without CYP51. Based on the 5′-end
In this study, the upstream regulatory region was essfully
nucleotide sequence of cyp51, the upstream region of cyp51 was
obtained by using the chromosome walking technique, based on 5'-end
obtained by chromosome walking technique to investigate the
sequence of cyp51 gene from Ustilago maydis