文档介绍：Detection DNA point mutation with rolling-circle
amplification chip
Lingwei Wu1, Yun Ling2 , Anshu Yang1, Shuixing Wang1 *
1 Jiangxi-OAI Joint Research Institute, Nanchang University
2 School of Pharmaceutical Science, Nanchang University
Nanchang 330047, China
* Corresponding author:shuixingw@
Abstract—We present a protocol with isothermal rolling-plementary sequences. However, Hydrogen bonding
amplification (RCA) to detect DNA point mutation on chip. The between target and probe hybrids is inherently weaker than
basic principle of the method is an allele-specific oligonucleotide covalent bonding, thus, making it more susceptible to
circularization mediated by special DNA ligase. The probe is breakage during stringent washes. To e this problem,
circularized when plementary sequences between Nilsson et. al.[2] synthesized a circularizable oligonucleotide
the probe oligonucleotide and target DNA. Mismatches around
the ligation site can prevent probe circularization. The probe (C-probe) for in situ target localization and detection.
circularized probe (C-probe) can be amplified by rolling circle Upon hybridization to a target DNA, the two ends of the
amplification to generate multimeric singlestranded DNA probe e juxtaposed and can be joined by DNA ligase.
(ssDNA) Under isothermal condition. There are sequence The C-probe was int