文档介绍：High electroporation efficiency of Corynebacterium
glutamicum with xenogeneic plasmid DNA
Ming Li*,Tao Niu,Jun-huan Zhang, Zhe Su,Dong-jun Kong
Tianjin Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of
Science & Technology, Tianjin 300457,China
Abstract—The restriction-modification systems of C. glutamicum In this study, we investigated the possibility of further
impede its uptake of foreign DNA and thus limiting its increasing the transformation efficiency of coryneform bacteria
transformation efficiency. Here we investigated the possibility of by characterizing and circumventing their restriction/
improving the transformation efficiency of C. glutamicum by
characterizing and circumventing their restriction/modification modification barrier, as well as by optimizing the
barrier, as well as by optimizing the conditions of electroporation conditions.
electroporation. We found that, among various conditions tested
for electroporation, the most important factor is the source of
II. MATERIALS AND METHODS
plasmid DNA. When plasmid DNA isolated from the dam--dcm-
E. coli mutant JM110 was used for transformation of C.
glutamicum that had been grown in BHIS supplemented with A. Bacterial strains, plasmids, and cultivation.
glycine % (w/v), INH (4 mg/ml) and tween80 % (w/v) at C.