文档介绍：Evaluation of DNA Oxidative Damage in Visceral
Cells Using 8-OHdG level
Wu Yang, Chang Qing, Yang Xu*
Laboratory of Environmental Sciences
College of Life Science, Huazhong Normal University
Wuhan 430079, China
*Corresponding author email: ******@nu.

Abstract—we developed a quantitative detection method that can present in DNA, is one of the most reliable and abundant
reflect the degree of visceral cell oxidative DNA damage induced markers of DNA damage because it reflects extremely low
by pound exposures using 8-OHdG as a levels of oxidative damage [4]. Previous studies demonstrated
biomarker in vitro. In this study formaldehyde (FA) was used as that urinary 8-OHdG levels are higher in smokers, cancer
a model pound and malondialdehyde (MDA) was patients, chronic renal failure patients, and semiconductor
used as a reference indicator. Rat liver cell suspension was
applied in this in vitro exposure experiments as testing material. workers with greater urinary arsenic and chromium exposure
The liver cells of rat were exposed to different concentrations of [5-7].
FA (0, 5, 15, 45μmol•L-1) for 1h, then the contents of 8-OHdG and 8-OHdG can pair with adenine and lead to G: C -T: A
MDA were measured. Research results showed that the 8-OHdG transversion mutations unless repaired before replication [8],
and MDA were increased gradually with