文档介绍：目录
一绪论................................................................01
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11荧光原理.......................................................01
12衍生化技术....................................................02
13流动相溶剂的选择.............................................04
14药物样品的前处理方法.......................................05
2羧酸衍生技术的简介............................................06
(荧光检测技术).........................06
3高校液相色谱的原理及简介....................................07
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二实验.................................................................13
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三结果与讨论................................................16
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四结论................................................................20
致谢....................................................................21
内容摘要
高效液相色谱(HPLC)是分析化学中发展最快、应用最广泛的领域之一,其分离特性使之成为临床化学、环境化学、生物学、毒物学等诸多领域中被广泛采用的有效分离和质量监控手段。由于化合物本身性质和检测手段的限制,并非所有物质都能够直接用于色谱分析,为此开发新型高灵敏衍生试剂,利用化学衍生化反应达到改变目标化合物的物理化学性质后,使之能够灵敏、快速和自动化测定是本文的研究目的。本文系统综