文档介绍：高速逆流色谱纯化红景天中的红景天甙 I V 4 p y 西南药业股份有限公司,重庆 400038 摘 要! d 3 D eEFh ’ I Q z . = # ? Y ’ I Q zDY 7 H d Y 7e^_ @ 1 H o m h I P $ N k @ , 9 > m h X Z Ol$ N 4 / _{|} A J} r S ? } . m 2 } , - Z D Sk H dtu e D a @ , 9 > m h . w Ol4 ’ I Q z Op- M ? m q , 4 : ~ Cef L2 m N ~ H d t5 e @ , 9 > m h $ N DL_ - " z @ O p ’ I Q z ? 4 { H ^ A() H 关键词! ’ I Q z 6 @ 1 H o m h 6 @ , 9 > m h 中图分类号: }L. ? 文献标识码: 0 文章编号: .--,O,- ?@+,-.q3-qO-.- ’O-q Study on the Purification of Salidroside by LI Yuan et al . (Southwest Pharmaceutical Co. ,Ltd. ,Chongqing 400038 ) Abstract [Objective] We do this experiment in order to study the way to extract salidroside. [Method] Salidroside was obtained by and HPLC with two phase solvent posed of chloroform !methanol "water ( 4 # $ 2 ) . [Result] The purity of salidroside could get % ,the coefficient of recovery could get %just after one purification. [Conclusion] This experiment gives a new way to prepare high- purity salidroside. Key words Salidroside ;High performance liquid chromatography ;High- speed counter- current chromalography 作者简介: I V+.LM-O 34P4 Q lU4 . ? L4hi t = 7R .S H 收稿日期: ,-.qO-.O-2 高速逆流色谱( High speed counter current chromatography , )是一种液-液色谱分离技术,它的流动相和固定相都是液体,这两种液体是互不相溶的 H 利用螺旋柱产生的离心力,使这互不相溶的两相溶剂系统不断混合,保留在管中, 先泵入的一相为固定相,得用恒流泵连续输入的为流动相, 随流动相进入管中的溶质在两相之间反复分配,按分配系数的大小,被依次洗脱,在流动相中分配比例大的先被洗脱出来 具有无不可逆吸附 r高回收率 r操作简便等优点,现今被越来越多地应用在天然产物的分离纯化中 H