文档介绍：作物学报 ACTA AGRONOMICA SINICA 2009, 35(11): 2022−2028 b/
ISSN 0496-3490; CODEN TSHPA9 E-mail: ******@.cn
DOI: .
Isolation and Characterization of Disease Resistance Gene Analogs from
Erianthus arundinaceus cDNA
QUE You-Xiong, XU Li-Ping*, LIN Jian-Wei, XU Jing-Sheng, ZHANG Mu-Qing*, and CHEN Ru-Kai
Key Laboratory of Sugarcane ic Improvement, Ministry of Agriculture / Fujian Agriculture and Forestry University, Fuzhou 350002, China
Abstract: Plant disease resistance genes (R-genes) encode some conserved motifs. According to the conserved motifs present in
the known NBS-LRR R-gene sequences and R gene analogs (RGAs), several degenerate primers were designed and applied in the
RGA isolation from Erianthus arundinaceus using PCR approach. In total, 6 RGAs were essfully obtained, with GenBank
accession numbers of EU685835, EU685836, EU685837, EU685838, EU685839, and EU685840. Multiple alignments showed
that the encoding sequences of the six clones were highly conserved and strikingly similar to the eleven most typical NBS-LRR
type R-gene peptide sequences, especially at the four NBS motifs of P-loop, kinase-2, kinase-3a, and HD. The identity percentage
at the amino-acid level ranged from % to % among all 17 sequences tested and from % to % among the six RGAs
cloned in this study. The results of cluster analysis and t