文档介绍：广西农业科学 2010,41(10):1029-1032
Guangxi Agricultural Sciences ·1029 ·
Germination and induction of axillary buds in micropropagated
Lonicerae hypoglauca
LI Kui-peng, HUANG Chang-yan, ZHOU Shan, YANG Mei-chun*
(Agricultural College, Guangxi University, Nanning 530005, China)
Abstract: The shoot of Lonicerae hypoglauca!cultivar (Lonicerae confusa DC) with axillary buds was used as explant
to study the effects of different sterilization methods and sampling seasons, and different concentrations of hormones and
active ingredient on the germination, induction and proliferation of axillary buds in micropropagated shoots. The sterilization
treatment with 75% alcohol for 25 bined with dipping shoots in % HgCl2 solution with tween-80 for 6 min was
found to be the most effective, as shown by % germination rate of axillary buds. Further, the sterilization effects were
found to be most effective in spring sampled explants, which germinated after 7 days of culture, compared to 12 days in
autumn samples. The suitable culture medium for germination and proliferation of axillary buds were found as follows: MS
media+6-BA ( mg/L) + KT( mg/L), and MS media+6-BA ( mg/L) + KT( mg/L), respectively.
Key words: L. hypoglauca; tissue culture; axillary bud; germination; proliferation
CLC number:+9 Document code:A Article:1002-8161(2010)10-1029-04
红腺忍