文档介绍：第 卷 第 期 集美大学学报 （自然科学版）
26 4 Ｓｐirｕlina ｐlatｅnsis
In this study, superoxide dismutase ( SOD) was isolated from , and then se-
quentially purified through ultrafiltration, two step salting-out method, DEAE ion exｃhange ｃhromatography and
TM
Superdex 200 gel filtration ｃhromatography. The results showed that the average speｃifiｃ of the enzyme was
4 131 U ／ mg, the purifiｃation multiple was 238. 96 and the extraｃtion reｃovery of this protein was 8. 3% in a
pilot sｃale system of 90 L ｃrude algal extraｃt. The result of SDS-PAGE analysis demonstrated that moleｃular
mass of SOD was 18. 4 ku. Repeat experiments showed similar results. In addition, the SOD aｃtivity of three re-
peated experiments was all greater than 4 000 U ／ mg.
Ｋｅｙ ｗｏｒｄｓ: Ｓｐirｕlina ｐlatｅnsis
; superoxide dismutase; separation; purifiｃation; pilot sｃale produｃtion
引言
0
螺旋藻起源于非洲和拉丁美洲[1] 在地球上存在的时间超过 亿 被称为自然界的活化石
， 35 a， 。
螺旋藻中蛋白质含量丰富[2 - 3] 是目前已知的理想蛋白质来源[4] 螺旋藻的早期研究也集中于此 近
， ， 。
年来 研究发现