文档介绍：摘要摘要目的:探讨自制脉冲电磁场治疗仪对兔激素型股骨头坏死模型的治疗作用。方法:本实验应用由加强的schwartzman反应的方法建立动物模型,选用 27只成年大耳白兔,耳缘静脉注射大肠杆菌内***(harides,LPS) 50ug/kg,24h后重复注射相同剂量LPS后,同时臀肌注射***强的松龙(Methylpoednisolone,MP)20mg/kg,间隔24h及48h后再注射两次相同剂量 MP,最后一次注射归后4周成功建立激素型股骨头缺血坏死模型。本实验采用由中国医学科学院生物医学工程研究所研制的脉冲电磁场治疗仪,其基本参数如下:波形为尖峰衰减波形;脉冲磁场频率为2~20Hz;最大磁场强度为10mT; 信号功率谱为0~25011z,主要功率频段为2~75Hz。建立模型过程中有9只实验动物死亡,将剩余18只实验动物随机分为2组,对照组9只,实验组为脉冲电磁场治疗组9只。实验组所有实验动物,接受电磁场治疗1小时/天,持续至实验结束。所有动物行一般状况评估、治疗后4周及8周时各选取4只及5只进行x线、MRI及标本的组织学病理检查。结果:^{P后4周,MRI显示骨髓腔内有不规则的低信号。 8周的x一线片示经脉冲电磁场治疗后骨小梁较对照组清晰,骨密度略有增加。MRI 示经脉冲电磁场治疗后骨髓腔内的不规则低信号较对照组模糊。组织病理学显示,对照组骨小梁变细,稀疏,结构紊乱,部分断裂:骨细胞核固缩,深染; 骨髓内造血组织减少,脂肪组织增多;空骨陷窝率增加(44±4%)。治疗组骨小梁增粗,致密,结构规则;表面新生骨形成:骨髓内造血组织增多;脂肪组织减少空骨陷窝率下降(28±4%)。结论:***强的松龙导致的早期股骨头缺血坏死模型及其治疗过程表现不明显,可能是激素所致骨髓细胞脂肪化是发展缓慢的病理过程。,抑制骨质疏松,一定程度控制激素型股骨头缺血坏死的发生和发展,进一步研究改进,可能成为治疗缺血性股骨头坏死的一种有效治疗方法。关键词:激素,股骨头坏岁E,脉冲电磁场 Abstracl The study on thetreatment ofpulse ic fields(PEMFs)on avascular necrosis offemoral head(AVFHN)induced by COrticoid inrabbit 0bject: 111epaper discussed themechanism ofthetreatment ic fields onavascular femoral headnecrosisinduced by corticoidinrabbit. Method: Thisexperiment applied theenforced Schwartzman reaction toestablishanimal model.、Ⅳechose wereinjected ofLPs 50ueJkg inear-edge 24hours,there wereinjected thesame quantity LPS would beinjected methylpoednisolone 20meCkg inthegluteus atthesame 24 hour and 48hour they were injected thesame quantity methylpoednisolone inthe other theanimal model ofavasculm femoral head necrosis induced by corticoidwas pulse ic fieldstreatment instrument was furnished by InstituteofBiomedical Engineering,the Chinese academy ofmedical basicparameter was as follows,the wave mark was aform ofpeak attenuation,it isfrequency was a 2-20 Hz,the maximal ic fields strength was 0 inT,thesignal posed ofthe0-250 Hz,the main power frequency se掣nent was 2- experiment had 9experimen