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酶联免疫吸附法检测与甲苯胺红不加热血清试验在梅毒检验中应用对比.doc

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酶联免疫吸附法检测与甲苯胺红不加热血清试验在梅毒检验中应用对比.doc

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酶联免疫吸附法检测与甲苯胺红不加热血清试验在梅毒检验中应用对比.doc

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文档介绍:酶联免疫吸附法检测与甲苯***红不加热血清试验在梅毒检验中应用对比【摘要】目的探讨酶联免疫吸附法( ELISA ) 检测与甲苯***红不加热血清试验( TRUST ) 在梅毒检验中应用对比情况。方法 500 例梅毒标本, 均采用 ELISA 与 TRUST 检测, 比较检测结果。结果 ELISA 血清阳性率高于 TRUST ( P< ), ELISA 检测结果的敏感性、特异性、阴性预测值、阳性预测值均高于 TRUST ( P< )。结论 ELISA 梅毒准确性较高, 适用于大批量样本筛查。【关键词】酶联免疫吸附法;甲苯***红不加热血清试验;梅毒;对比 DOI : -5547/ parison between enzyme linked immunosorbent assay and toluidine red unheated serum reagin test in syphilis detection LIANG Fu-quan , ZHU Xiu-lan , PENG Jian-hua. Department of Inspection , Dongguan Third People ’s Hospital , Dongguan 523320 , China 【 Abstract 】 Objective To investigate application of enzyme linked immunosorbent assay ( ELISA ) and toluidine red unheated serum reagin test ( TRUST ) in syphilis detection. Methods A total of 500 syphilis samples were inspected by ELISA and TRUST , and their detection e pared. Results ELISA showed higher serum positive rate than TRUST ( P< ), and its sensibility , specificity , negative and positive predictive value were all higher than those of TRUST ( P< ). Conclusion Due to high accuracy in syphilis detection , ELISA is suitable for large sample screening. 【 Key words 】 Enzyme linked immunosorbent assay ; Toluidine red unheated serum reagin test ; Syphilis ; Comparison 梅毒属于性传播疾病的一种, 随着我国改革开放的发展, 婚育观念的变化, 梅毒发生率呈现明显增高的趋势[1, 2]。梅毒是由于梅毒螺旋体感染造成的, 其传播途径可以分为性传播和母婴传播, 妊娠梅毒可能造成流产、死胎、胎儿畸形等严重的并发症, 因而对于梅毒进行快速、准确的诊断成为