文档介绍：Establishment and Maintenance of DNA Methylation Patterns in Mouse Ndn: Implications for Maintenance of Imprinting in Target Genes of the Imprinting Center
Meredith L. Hanel and Rachel Wevrick
Speaker: Jianhuan Chen
Major:biotechnology
ID:00274022
Date:, 2002
Ndn:
    Ndn is located on chromosome 7C, an imprinted region of the mouse genome. Imprinting of Ndn and adjacent paternally expressed genes is regulated by a regional imprinting control element known as the imprinting center (IC).
    Ndn is an ideal gene for imprinting study because it has a simple, intronless gene structure and is a target of the IC rather than being intimately involved in imprinting other genes.
Introduction
    Imprinting : a form of gene regulation whereby certain genes are restricted in expression to only one parental allele
    ic modifications such as DNA methylation, which occur in gametes and can be stably propagated, are presumed to establish and maintain the imprint in target genes.
MATERIALS AND METHODS
Collection of oocytes, early embryos, and sperm.
DNA extraction.
SBS.
PCR.
Cloning and sequencing.
Genomic structure analysis.
Collection of oocytes, early embryos, and sperm.
    Early embryos and gametes were collected from C57BL/6 mice
    Some of the blastocysts were derived from crosses between C57BL/6 females and Mus spretus (SPRET) males.
DNA ext