文档介绍：2
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( Institute of Environment and Sustainable Development in Agriculture, Chinese
Academy of Agricultural Sciences, Beijing 100081, China)
Abstract: A 180 L culture vessel for sugar free tissue culture with new type CO2 releasing p ipe and its environment
control system were designed, aim ing at solving the p roblem s existing in conventional p lant tissue culture and from the
view point of p racticing p lant sugar free m icrop ropagation. By combining the three techniques of small flow control,
three way valve adjustment and PWM control pattern, the CO2 concentration in side of culture vessel was controlled
within ± 50 μmol· mol- 1 around the target value. The technology of combining membrane tectoria with gas cycle
adsorp tion was used to realize the auto control of inner relative hum ility and the control p recision has reached ± 2%.
The result of experiment on culturing crabapp le p lant sugar free tissue indicates that this system has the function of
promoting the growth of tissue culture plantlets and improving their physiological quality.
Key words: p lant sugar free m icrop ropagation; cultural vessel; environmental control system