文档介绍：中国化工仪器网版权所有未经书面授权不得以任何形式进行复制血水草生物碱的分离作者左雄军李静建立了内衬聚亚***型C18反相高效液相柱分离血水草生物碱HPLC 新方法考察了梯度洗脱分离时起始缓冲液组成及pH 血水草,生物碱,高效液相色谱法 HPLC Separation of Alkaloids in Eomecon Chionantha HanceZuo Xiongjun, Li Jing (Guangzhou Institute of Chemistry, Chinese Academy of Sciences,Guangzhou 510650) Abstract A new reverse - phase HPLC method for the separation of quaternary alkaloids in Eomecon chionantha Hance is described. A C18 col umn wi th silica - based packing pre - shielded with polyimide is employed. The effects of initial position and pH on the gradient elution have been investigated. The major alkaloids can be separated from minor constituents under a linear gradient elution mode. The initial eluate is mol/L K2HPO4 at pH , whereas the final eluate is 100 methanol. It is found that the contents of 中国化工仪器网版权所有未经书面授权不得以任何形式进行复制alkaloids in the herb from different places are different, and vary with growing seasons too. Keywords Eomecon chionantha Hance, Alkaloid, HPLC 血水草系***科白屈菜属植物包含多种季***型生物碱如血根碱白屈菜红碱普洛托品碱及别隐品碱等对该属植物生物碱曾尝试过用纸层析法[1]薄层层析法[2]离子对色谱法[3]等分离分析但分离中或多或少存在着一些问题难以获得准确的定性定量结果毛细管等速电泳[4]和高效液相色谱法[5]为该类生物碱的分析提供了快速准确的手段但用HPLC 分离时仍存在线性响应太差峰拖尾洗脱液组成复杂等问题[5]优化生物碱的HPLC 分离条件仍是值得探讨的课题本工作用自己研制的内衬聚亚***型反相高效液相色谱柱[6]考察了血水草生物碱的分离1 仪器 Waters 991 型HPLC