文档介绍:) 36À) 22Á ) Å Æ # % & & Ç Vol.36,No.22
ecellmorphologyandtheexpressionof
epithelialmesenchymaltransition(EMT)markerproteinsafterMCF7cellsacquiredtamoxifenresistance.
Methods Thecellmorphologywasrevealedthroughimmunofluorescentstaining.TheexpressionofEMT
markerproteinswasdetectedbyquantitativerealtimePCR(qRTPCR)andWesternblotting.Transwellassay
wasusedtodetectthedifferenceofcellmigration.PI3KAktandMAPK/Erksignalingpathwayswereevaluated
inMCF7cellsandtamoxifenresistantMCF7Rcells.ThemigrationandtheexpressionofEMTmarkerproteins
werereevaluatedafterinhibitingthesignalingpathways.Results IntheMCF7cells,intercellularcontact
wascloseandEcadherinwaspredominantlylocalizedinthemembrane.However,cellcontactbecameloose
andEcadherinwasalmosttranslocatedtothecytoplasminthetamoxifenresistantMCF7Rcells.Themigration
wasenhancedandtheexpressionofvimentinandfibronectinwasincreasedwhentheMCF7cellsacquired
tamoxifenresistance(P<005).PI3KAktandMAPK/Erksignalingpathwayswereabnormallyactivatedin
thetamoxifenresistantMCF7R cells.Meanwhile,themigrationandtheexpressionofmesenchymalmarker
proteinsweresignificantlysuppressedwheninhibitingPI3KAktpathwayratherthanMAPK/Erkpathway(P<
005).Conclusion EMTmaycontributetotheincreasedmigrationoftamoxifenresistantb