1 / 5
文档名称:

人乳腺癌MCF-7他莫昔芬耐受细胞株EMT表型的鉴定及其机制的初步研究.pdf

格式:pdf   大小:1,687KB   页数:5页
下载后只包含 1 个 PDF 格式的文档,没有任何的图纸或源代码,查看文件列表

如果您已付费下载过本站文档,您可以点这里二次下载

分享

预览

人乳腺癌MCF-7他莫昔芬耐受细胞株EMT表型的鉴定及其机制的初步研究.pdf

上传人:q1188830 2022/2/20 文件大小:1.65 MB

下载得到文件列表

人乳腺癌MCF-7他莫昔芬耐受细胞株EMT表型的鉴定及其机制的初步研究.pdf

相关文档

文档介绍

文档介绍:) 36À) 22Á ) Å Æ # % & & Ç Vol.36,No.22
           ecellmorphologyandtheexpressionof
epithelialmesenchymaltransition(EMT)markerproteinsafterMCF7cellsacquiredtamoxifenresistance.
Methods  Thecellmorphologywasrevealedthroughimmunofluorescentstaining.TheexpressionofEMT
markerproteinswasdetectedbyquantitativerealtimePCR(qRTPCR)andWesternblotting.Transwellassay
wasusedtodetectthedifferenceofcellmigration.PI3KAktandMAPK/Erksignalingpathwayswereevaluated
inMCF7cellsandtamoxifenresistantMCF7Rcells.ThemigrationandtheexpressionofEMTmarkerproteins
werereevaluatedafterinhibitingthesignalingpathways.Results IntheMCF7cells,intercellularcontact
wascloseandEcadherinwaspredominantlylocalizedinthemembrane.However,cellcontactbecameloose
andEcadherinwasalmosttranslocatedtothecytoplasminthetamoxifenresistantMCF7Rcells.Themigration
wasenhancedandtheexpressionofvimentinandfibronectinwasincreasedwhentheMCF7cellsacquired
tamoxifenresistance(P<005).PI3KAktandMAPK/Erksignalingpathwayswereabnormallyactivatedin
thetamoxifenresistantMCF7R cells.Meanwhile,themigrationandtheexpressionofmesenchymalmarker
proteinsweresignificantlysuppressedwheninhibitingPI3KAktpathwayratherthanMAPK/Erkpathway(P<
005).Conclusion EMTmaycontributetotheincreasedmigrationoftamoxifenresistantb