文档介绍：: .
分子植物育种 密码子使用偏好性的影响较之其它位点更为重要；除自然选择之外，密码子偏好性还受到突变压力和核苷
酸组成等因素的影响；共有 16 个密码子被鉴定为金花茶叶绿体基因组最优密码子。本研究内容为金花茶
叶绿体基因组的遗传修饰和系统发育研究奠定了基础。
关键词 金花茶; 叶绿体基因组; 密码子偏好性; 最优密码子
Codon Usage Bias Analysis of Chloroplast Genome in Camellia
nitidissima
Geng Xiaoshan Jia Wei Chen Jianing He Caifang Zhu Yulin * Liu Qin *
College of Biology and Pharmacy of Yulin Normal University, Yulin, 537000* Corresponding author, ******@; ******@
Abstract In order to reveal the codon usage bias and to screen the optimal codon suitable for Camellia
nitidissima chloroplast expression, the codon usage patterns of 52 coding genes (coding sequence, CDS) in
Camellia nitidissima chloroplast genome are studied by using software such as CodonW, Microsoft and R
language. The results show that the use of synonymous codons in the chloroplast genome of Camellia nitidissima
preferred to end with A/U, especially with U. The effective number of codon (ENC) is greater than 35, which is a
weak preference; the base composition of codon position 3 is significantly different from positions 1 and 2, and
the base composition of this position have an important influence on codon usage bias. In addition to natural
selection, codon usage bias is also influenced by mutational pressure and base composition; 17 codons are
identified as optimal codons in the chloroplast genome of Camellia nitidissima. This study lays the foundation for
the genetic modification and phylogenetic study of the chloroplast genome of Camellia nitidissima.
Keywords Camellia nitidissima; Chloroplast genome; Codon usage bias; Optimal codons
三联密码子是遗传信息从 mRNA