文档介绍：: .
中国中药杂志 UPLC-Q-TOF-MS/MS 技术，借助 UNIFI 软件对保留时间、一级、二级质谱信息等
进行分析，初步鉴定了小儿豉翘清热颗粒中的 11 个苯乙醇苷类组分，并以小儿豉翘清热颗粒给药后幼龄大鼠血浆中
的化学成分作为研究对象，从血浆样本中鉴定出 69 个苯乙醇苷相关代谢产物。此外，该研究模拟儿童体内肠道菌群
转化体系，探讨肠道菌群对苯乙醇苷类组分中代表性成分连翘酯苷 A、连翘酯苷 E、红景天苷的代谢影响，建立了小
儿食积胃肠积热模型，以研究苯乙醇苷类组分的差异性代谢产物。通过对代表性成分连翘酯苷 A、连翘酯苷 E、红景
天苷入血成分及肠菌转化产物之间的对比分析，发现主要代谢途径为脱氢、氧化、乙酰化、硫酸化和葡萄糖醛酸化反
应。研究结果揭示了苯乙醇苷类组分在胃肠道的转化规律，通过初步探讨药理作用机制，为进一步阐明小儿豉翘清热
颗粒药效物质基础提供新的认识，对儿科中药复方的研究具有一定的理论参考作用。
关键词 小儿豉翘清热颗粒；苯乙醇苷；代谢；肠道菌群；UPLC-Q-TOF-MS
DOI: .
Phenylethanoid glycosides in Xiaoer Chiqiao Qingre granules and its metabolic
transformation in vitro and in vivo
WEN Ling1 , ZHU Mao-mao1 , YANG Yan-jun1 , ZHU Xiang-jun2 , LIU Jun3 , ZHAO Jing3 , FENG Liang1 *, JIA Xiao-bin1 *
( Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China;
Health Development Research Center, National Health and Family Planning Commission Contraceptives Adverse Reaction Surveillance
Center, Nanjing 210000, China; Key Laboratory of Chinese Medicine and Characteristic Preparations for Paediatrics, Jumpcan
Pharmaceutical Group Co. ,Ltd., Taixing 225400, China)
Abstract To clarify the metabolic transformation mechanism of phenylethanoid glycosides in Xiaoer Chiqiao Qingre
Granules in vivo, this study extracted and separated the phenylethanoid glycosides in Xiaoer Chiqiao Qingre Granules. Based
on UPLC-Q-TOF-MS/MS technology, the retention time and primary and secondary mass spectrometry information were
analyzed by UNIFI software, and 11 phenylethanoid glycosides in Xiaoer Chiqiao Qin