文档介绍：1 延胡索产地醋煮工艺的研究作者:田永亮,窦志英,曹柳,王萍,李迎春【摘要】目的优选出延胡索最佳产地醋煮工艺。方法首先对温度、用醋量和拌润时间进行了单因素考察, 然后采用正交实验, 以用醋量、拌润时间和粒度作为正交设计因素, 再采用 HPLC 法测定延胡索中延胡索乙素、原阿片碱和去氢紫堇碱 3 种有效成分的含量, 最后获得延胡索最佳产地醋煮工艺。结果延胡索最佳产地醋煮工艺即选小个子药材加 40% 的醋和适量水拌润 4h 后煮至醋液吸尽;大个药材应适当破碎后再炮制。结论优选出的延胡索最佳产地醋煮工艺简单易操作,稳定可行。【关键词】产地加工; 醋煮工艺; 延胡索; 延胡 Abstract : Objective To optimize the origin-boiling with vinegar technology. MethodsSingle-factor experiment was carried out with temperature, vinegar volume and mixing time at first, orthogonal experiment was carried out with vinegar 2 volume, mixing time and particle size as factors of the orthogonal experiment design. HPLC was used to determine the contents of tetrahydropalmatine, protopine and dehydrocorydaline which were the three main active ingredients in Rhizoma Corydalis. At last, the optimum origin- boiling with vinegar technology of Rhizoma Coridalis was obtained. ResultsThe optimum origin- boiling with vinegar technology of Rhizoma Coridalis was that the small Corydalis was added 40% vinegar and infiltrated 4h and boiled, the big ones was processed after optimum origin- boiling with vinegar technology of Rhizoma Coridalis is simple, easy and stably. Key words : Origin-processing; Boiling with vinegar technology; Corydalis tuber; Tetrahydropalmatine; Protopine; Dehydrocorydaline 延胡索为罂粟科(Papaveraceae) 植物延胡索 Corydalis yanhusuo 的干燥块茎。具有活血、利气、止痛之功效, 临床用于治疗胸胁、脘腹疼痛、经闭痛经、产后瘀阻、跌扑肿痛等症[ 1] 。延胡索中主要有效成分为生物碱, 在药材中多数以结合态的生物碱盐存在, 少数碱性极弱的以 3 游离态形式存在[2]。由于市售的延胡索都是经过产地水煮加工并经炮制后作为临床药用的, 这样工序较繁琐, 质量难以控制和保证。在产地加工过程中, 延胡索水煮后损失了很多有效成分, 有学者调研时发现( 毛淑杰, 游修琪, 顾雪竹. 产地不同采制方法对延胡索质量的影响. 中华中医药学会炮制分会 2008 年学术研讨会论文集, 2008 : 210. ) ,当地农民有用其水煮液治病的, 而且疗效很好。根据本实验室对不同炮制方法对延胡索中有效成分含量影响[3] 的考察结果, 延胡索鲜品经醋煮后的水提液中, 其主要有效成分延胡索乙素、原阿片碱和去氢紫堇碱的含量也较高。因而本实验选择了鲜品醋煮作为产地加工炮制一体化的工艺,以延胡索乙素、原阿片碱和去氢紫堇碱作为指标, 先进行了对烘制温度、用醋量和拌润时间的单因素考察,再通过采用正交实验法, 以用醋量、拌润时间和粒度为考察因素, 优选出延胡索产地醋煮工艺的最佳条件, 以期为产地加工与炮制一体化工艺的进一步研究奠定基础。 1 仪器与试药 DHG78-1 烘箱( 湖北省黄石市医疗器械厂); BP121S 电子天平(瑞士托利斯) ; X205 红外感应天平(十万分之一, METTLER TOLEDO ); A