电磁场对绝经后大鼠骨质疏松的影响.doc

文档介绍：1 电磁场对绝经后大鼠骨质疏松的影响作者:黄素然,罗二平,申广浩,黎普茜,王静文,周倩【关键词】电磁场;骨质疏松;骨密度;骨钙素 Effects of ic fields on osteoporosis in ovariectomized rats 【 Abstract 】 AIM: To study the effects of ic fields (EMFs) on osteoporosis in ovariectomized (postmenopausal) rats. METHODS: The osteoporotic models were established in 20 female SD rats by bilateral ovariectomy and then divided into 2 groups (n=10). The experimental group was exposed to 15 Hz EMFs (8× 10-4 T,6h / d), and the control group was not exposed to EMFs. All the animals were raised in the same conditions and killed at 12 th week after operation. The changes in serum bone carboxyglutamic acidcontaining protEin (BGP), bone mineral density(BMD) and biomechanical properties were examined. 2 RESULTS: Compared with control group, BMD, maximum load and structural rigidity in experimental group were significantly increased ( ), and serum BGP were decreased obviously ( ). CONCLUSION: EMFs is helpful to the treatment and prevention of osteoporosis. 【 Keywords 】 ic fields; osteoporosis; bone density; osteocalcin 【摘要】目的: 观察电磁场(EMFs) 对去势( 绝经) 后大鼠骨质疏松症的影响. 方法: 20 只雌性 SD 大鼠行双侧卵巢切除术建立骨质疏松动物模型, 随机分为两组( n=10 ): 实验组每日电磁场辐照 6 h(8 × 10-4 T, 15 Hz) , 对照组饲养环境相同, 但不暴磁. 12 wk 后, 对血清骨钙素、骨密度以及生物力学等参数进行检测. 结果: 与对照组相比, 实验组骨密度、最大载荷及结构刚度均有增加( ), 血清骨钙素(BGP) 水平减少( ). 结论: EMFs 有益于骨质疏松症的预防和治疗. 【关键词】电磁场;骨质疏松;骨密度;骨钙素 0 引言 3 随着生物医学工程学的快速发展,电磁场( ic fields , EMFs ) 作为一种可施加于人体的外来物理因子, 对其热及非热生物效应的研究已成为国内外生物工程学界的研究热点[ 1-3 ]. EMFs 是一种高能非电离辐射, 人体对一定频率、一定强度的电磁场高度敏感, 机体受到刺激后, 通过下丘脑垂体肾上腺系统产生效果, 对皮质生物电活动、内分泌乃至整个机体产生调节功能. EMFs 在骨科领域已用于治疗假关节、骨不连或延迟愈合、新鲜骨折、骨移植、骨关节炎等, 近年来又被用来预防和治疗骨质疏松症[ 4-5 ]. 我们利用低强度 EMFs ,通过建立骨质疏松动物模型,探讨低强度 EMFs 对骨质疏松症的影响,以期为骨质疏松症的预防和治疗提供新的思路和理论依据. 1 材料和方法 材料 仪器 EMFs 发生仪( GZY 型) 由第四军医大学生物医学工程系研制(专利号: ) ,均匀场的产生采用 mm 漆包铜线绕制亥姆霍兹线圈对(半径 R=400 mm ,线圈匝数 N=800 ) 4 图1 亥姆霍兹线圈略在轴线上任意一点 P (图 1 )出的磁感应强度为: B(z)= μ 0NIR2/2{1/(R2+z2)3/2+1/ [ R2+(z-R)2 ] 3/2} 式中,第一项为左边线圈的贡献; z=R/2 点来说, 公轴线中点处 B 的值: B(R/2)=8 μ 0NI/53/2.