文档介绍：1 高效液相色谱法测定磷酸川芎嗪纳米脂质体中磷酸川芎嗪的含量【摘要】目的建立 HPLC 法测定磷酸川芎嗪纳米脂质体中磷酸川芎嗪的含量。方法色谱柱为 phenomenex 飡 Luna C18(2)100A( mm × 250 mm,5 μ m) 柱, phenomenex 飡保护柱; 流动相为甲醇-水(3∶ 2) ;流速 ml/min ;检测波长 295 nm ; 柱温为室温。结果磷酸川芎嗪与辅料及溶剂峰分离良好,在 ~ μ g/ml 浓度范围内线性关系良好(r= 9) 。结论该方法简便易行,准确可靠,可用于磷酸川芎嗪纳米脂质体的含量测定。【关键词】磷酸川芎嗪纳米脂质体高效液相色谱 Abstract : ObjectiveTo establish an HPLC method for the determination of the content of tetramethylpyrazine phosphate (TMPP) in TMPP nanoliposome. MethodsThe condition of HPLC was as follows: phenomenex 飡 Luna C18(2)100A column( mm × 250 mm,5 μ m), mobile phase: methanol/water (3∶ 2) and wavelength:295 nm. The flow rate was ml/min 2 and the column temperature was room temperature. ResultsThere was good relation between TMPP and the excipients under this chromatographic condition . The calibration curve was linear in the range of ~ μ g/ml(r= 9).ConclusionThe method is simple and accurate. It can be used for the determination of content of tetramethylpyrazine phosphate nanoliposome . Key words : Tetramethylpyrazine phosphate(TMPP); Nanoliposome; HPLC 川芎嗪(Tetramethylpyrazine , TMP) 是中药川芎中的主要活性生物碱,临床上广泛用于缺血性脑血管病的治疗。已上市的川芎嗪制剂主要有磷酸川芎嗪(Tetramethylpyrazine phosphate , TMPP) 片剂、胶囊剂和注射剂[1]。口服制剂生物利用度不高; 注射剂给药不方便。有研究报道, 川芎嗪鼻腔吸收良好[2]。作者拟将川芎嗪研制成纳米脂质体进行鼻腔给药, 以期为川芎嗪的临床应用提供一个方便有效的新给药系统。为控制其质量, 本研究采用 HPLC 法测定磷酸川芎嗪纳米脂质体中磷酸川芎嗪含量。 1 仪器与试药 3 仪器 Summit P680 型高效液相色谱仪