文档介绍：1 多囊卵巢综合征患者脂肪组织 TNFR2mRNA 的表达及其意义作者:王竹晨顾熊飞杨冬梓邝健全【关键词】多囊卵巢【摘要】目的研究多囊卵巢综合征( PCOS ) 脂肪组织肿瘤坏死因子受体Ⅱ( TNFR2 ) mRNA 的表达, 从分子水平探讨 PCOS 患者发生胰岛素抵抗( IR )的分子生物学机制。方法采用 RT-PCR 技术结合内对照,半定量检测 PCOS 及其对照组脂肪组织 TNFR2mRNA 的表达。结果 TNFR2mRNA 的表达量在 PCOS 肥胖组( ± , ) 和 PCOS 非肥胖组( ± , ) 均显著大于非肥胖对照组( ± ), 且以 PCOS 肥胖组升高更为明显( ), PCOS 肥胖组与肥胖对照组( ± )相比则差异无显著性( ) 。结论 PCOS 两组脂肪组织 TNFR2mRNA 的表达均较非肥胖对照组增强,且以 PCO S 肥胖组升高更为明显, 高水平的肿瘤坏死因子α( TNF α)可能通过上调 TNFR2 促进 PCOS 者 TNF α系统活性增强。 2 关键词多囊卵巢综合征胰岛素抵抗肿瘤坏死因子受体Ⅱ mRNA 肿瘤坏死因子α.【 Abstract 】 Objective We study the expression of TNFR2mRNA in adipose tissue from PCOS to get insight into the mechanism of IR in PCOS at the molecular biological We survey the expression of TNFR2mRNA in adipose tissue by semi-quantitative RT-PCR technique with inner- The expression of TNFR2mRNA inadipose from the obese PCOS group ( ± , ) and the nonobese PCOS group ( ± , ) is markedly higher than the nonobese control group ( ± ), and the obese PCOS group presents more increase ( ) .The difference between the obese PCOS group and the obese control group ( ± ) is of no statistically significance ( ) .Conclusion These results suggest that the expression of TN ˉ FR2mRNA in adipose tissue from PCOS patient isabnormally high TNF α c