文档介绍：MethodsMethods inin MolecularMolecular BiologyBiologyTM
VOLUME 248
AntibodyAntibody
EngineeringEngineering
MethodsMethods andand ProtocolsProtocols
Edited by
Benny K. C. Lo
1
Resources for the Antibody Engineer
Benny K. C. Lo and Yu Wai Chen
1. Introduction
The contains a wealth of information and tools that are relevant to
various aspects of antibody engineering. Here, we present a collection of use-
ful websites and software that is specific to antibody structure analysis and
engineering, as well as for general protein analysis. Although this survey is by
no plete, it represents a good starting point. This list is accurate at
the time of writing (August 2003).
2. List of Websites
. Antibody-Specific Sites
. The Kabat Database (G. Johnson and , 2002;
)
Created by E. A. Kabat and T. T. Wu in 1966, the Kabat database pub-
lishes aligned sequences of antibodies, T-cell receptors, major pati-
plex (MHC) class I and II molecules, and other proteins of
immunological interest. A searchable interface is provided by the SeqhuntII
tool, and a range of utilities is available for sequence alignment, sequence
subgroup classification, and the generation of variability plots (see Chapter 2
for more details).
. KabatMan (A. C. R. Martin, 2002;
/abs/)
This is a web interface to make simple queries to the Kabat sequence data-
base. For plex cases, queries should be sent directly in the KabatMan
SQL-like query language.
From: Methods in Molecular Biology, Vol. 248: Antibody Engineering: Methods and Protocols
Edited by: B. K. C. Lo © Humana Press Inc., Totowa, NJ
3
4 Lo and Chen
. IMGT, the International ics Information System®
(M. -, 2002; )
IMGT is an integrated information system that specializes in antibodies, T-
cell receptors, and MHC molecules of all vertebrate species. It provides -
mon portal to standardized data that include nucleotide and protein sequences,